Hyperpolarized ¹³C Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of l-Lactate in Vivo.

ABSTRACT: The dynamics of l-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized ¹³C magnetic resonance spectroscopy (MRS). Following the intravenous injection of hyperpolarized [1-¹³C]lactate, we observed that the distribution of the ¹³C label between lactate and pyruvate, which has been shown to be representative of their pool size ratio, is different in NMRI and C57BL/6 mice, the latter exhibiting a higher level of cerebral lactate dehydrogenase A ( Ldha) expression. On the basis of this observation, and an additional set of experiments showing that the cerebral conversion of [1-¹³C]lactate to [1-

SUBMITTER: Takado Y 

PROVIDER: S-EPMC6119468 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature