A Microsphere-Supported Lipid Bilayer Platform for DNA Reactions on a Fluid Surface.

ABSTRACT: We report a versatile microsphere-supported lipid bilayer system that can serve as a general-purpose platform for implementing DNA nanotechnologies on a fluid surface. To demonstrate our platform, we implemented both toehold-mediated strand displacement (TMSD) and DNAzyme reactions, which are typically performed in solution and which are the cornerstone of DNA-based molecular logic and dynamic DNA nanotechnology, on the surface. We functionalized microspheres bearing supported lipid bilayers (μSLBs) with membrane-bound nucleic acid components. Using functionalized μSLBs, we developed TMSD and DNAzyme reactions by optimizing reaction conditions to reduce nonspecific interactions between DNA and phospholipids and to enhance bilayer stability. Additionally, the physical and optical properties

SUBMITTER: Fabry-Wood A 

PROVIDER: S-EPMC6119471 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature