Conserved temporal ordering of promoter activation implicates common mechanisms governing the immediate early response across cell types and stimuli.

ABSTRACT: The promoters of immediate early genes (IEGs) are rapidly activated in response to an external stimulus. These genes, also known as primary response genes, have been identified in a range of cell types, under diverse extracellular signals and using varying experimental protocols. Whereas genomic dissection on a case-by-case basis has not resulted in a comprehensive catalogue of IEGs, a rigorous meta-analysis of eight genome-wide FANTOM5 CAGE (cap analysis of gene expression) time course datasets reveals successive waves of promoter activation in IEGs, recapitulating known relationships between cell types and stimuli: we obtain a set of 57 (42 protein-coding) candidate IEGs possessing promoters that consistently drive a rapid but transient increase in expression over time. These genes show

SUBMITTER: Vacca A 

PROVIDER: S-EPMC6119861 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature