Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns.

ABSTRACT:

Background

Proteins within the cytoplasmic membrane display distinct localization patterns and arrangements. While multiple models exist describing the dynamics of membrane proteins, to date, there have been few systematic studies, particularly in bacteria, to evaluate how protein size, number of transmembrane domains, and temperature affect their diffusion, and if conserved localization patterns exist.

Results

We have used fluorescence microscopy, single-molecule tracking (SMT), and computer-aided visualization methods to obtain a better understanding of the three-dimensional organization of bacterial membrane proteins, using the model bacterium Bacillus subtilis. First, we carried out a systematic study of the localization of over 200 B. subtilis membrane proteins, tagged

SUBMITTER: Lucena D 

PROVIDER: S-EPMC6120080 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature