Activation of Smoothened in the Hedgehog pathway unexpectedly increases Gα_s-dependent cAMP levels in Drosophila.

ABSTRACT: Hedgehog (Hh) signaling plays a key role in the development and maintenance of animal tissues. This signaling is mediated by the atypical G protein-coupled receptor (GPCR) Smoothened (Smo). Smo activation leads to signaling through several well-characterized effectors to activate Hh target gene expression. Recent studies have implicated activation of the heterotrimeric G protein subunit Gα_i and the subsequent decrease in cellular cAMP levels in promoting the Hh response in flies and mammals. Although Hh stimulation decreases cAMP levels in some insect cell lines, here using a bioluminescence resonance energy transfer (BRET)-based assay we found that this stimulation had no detectable effect in Drosophila S2-R+ cells. However, we observed an unexpected and significant Gα

SUBMITTER: Praktiknjo SD 

PROVIDER: S-EPMC6120187 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature