Distinct requirements of linker DNA and transcriptional activators in promoting SAGA-mediated nucleosome acetylation.

ABSTRACT: The Spt-Ada-Gcn5 acetyltransferase (SAGA) family of transcriptional coactivators are prototypical nucleosome acetyltransferase complexes that regulate multiple steps in gene transcription. The size and complexity of both the SAGA enzyme and the chromatin substrate provide numerous opportunities for regulating the acetylation process. To better probe this regulation, here we developed a bead-based nucleosome acetylation assay to characterize the binding interactions and kinetics of acetylation with different nucleosomal substrates and the full SAGA complex purified from budding yeast (Saccharomyces cerevisiae). We found that SAGA-mediated nucleosome acetylation is stimulated up to 9-fold by DNA flanking the nucleosome, both by facilitating the binding of SAGA and by accelerating acet

SUBMITTER: Mittal C 

PROVIDER: S-EPMC6120209 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature