Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia.

ABSTRACT: Phosphatidylserine (PS) exposure increases as red cells age, and is an important signal for the removal of senescent cells from the circulation. PS exposure is elevated in red cells from sickle cell anaemia (SCA) patients and is thought to enhance haemolysis and vaso-occlusion. Although precise conditions leading to its externalisation are unclear, high intracellular Ca²⁺ has been implicated. Red cells from SCA patients are also exposed to an increased oxidative challenge, and we postulated that this stimulates PS exposure, through increased Ca²⁺ levels. We tested four different ways of generating oxidative stress: hypoxanthine and xanthine oxidase, phenazine methosulphate, nitrite and tert-butyl hydroperoxide, together with thiol modification with N-ethylmaleimide (N

SUBMITTER: Hannemann A 

PROVIDER: S-EPMC6120535 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature