Bronchiolitis obliterans syndrome susceptibility and the pulmonary microbiome.

ABSTRACT:

Background

Lung transplantation outcomes remain complicated by bronchiolitis obliterans syndrome (BOS), a major cause of mortality and retransplantation for patients. A variety of factors linking inflammation and BOS have emerged, meriting further exploration of the microbiome as a source of inflammation. In this analysis, we determined features of the pulmonary microbiome associated with BOS susceptibility.

Methods

Bronchoalveolar lavage (BAL) samples were collected from 25 patients during standard of care bronchoscopies before BOS onset. Microbial DNA was isolated from BAL fluid and prepared for metagenomics shotgun sequencing. Patient microbiomes were phenotyped using k-means clustering and compared to determine effects on BOS-free survival.

Results

Clustering iden

SUBMITTER: Schott C 

PROVIDER: S-EPMC6120773 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature