Cigarette Smoke Induces Stem Cell Features of Pancreatic Cancer Cells via PAF1.

ABSTRACT:

Background & aims

Cigarette smoking is a major risk factor for pancreatic cancer. Aggressive pancreatic tumors contain cancer cells with stem cell features. We investigated whether cigarette smoke induces stem cell features in pancreatic cancer cells.

Methods

Kras^G12D; Pdx1-Cre mice were exposed to cigarette smoke or clean air (controls) for up to 20 weeks; pancreata were collected and analyzed by histology, quantitative reverse transcription polymerase chain reaction, and confocal immunofluorescence microscopy. HPNE and Capan1 cells were exposed to cigarette smoke extract (CSE), nicotine and nicotine-derived carcinogens (NNN or NNK), or clean air (controls) for 80 days and evaluated for stem cell markers and features using flow cytometry-based autofluorescence, s

SUBMITTER: Nimmakayala RK 

PROVIDER: S-EPMC6120776 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature