Selection and Application of Tissue microRNAs for Nonendoscopic Diagnosis of Barrett's Esophagus.

ABSTRACT:

Background & aims

MicroRNA (miRNA) is highly stable in biospecimens and provides tissue-specific profiles, making it a useful biomarker of carcinogenesis. We aimed to discover a set of miRNAs that could accurately discriminate Barrett's esophagus (BE) from normal esophageal tissue and to test its diagnostic accuracy when applied to samples collected by a noninvasive esophageal cell sampling device.

Methods

We analyzed miRNA expression profiles of 2 independent sets of esophageal biopsy tissues collected during endoscopy from 38 patients with BE and 26 patients with normal esophagus (controls) using Agilent microarray and Nanostring nCounter assays. Consistently up-regulated miRNAs were quantified by real-time polymerase chain reaction in esophageal tissues collected by Cytos

SUBMITTER: Li X 

PROVIDER: S-EPMC6120784 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature