A General Protein <i>O-</i>Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen <i>Tannerella forsythia</i>: <i>O</i>-Glycan Biosynthesis and Immunological Implications.

ABSTRACT: The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins. The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity. To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic. Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in comb

SUBMITTER: Tomek MB 

PROVIDER: S-EPMC6120980 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature