Prolactin Variants in Human Pituitaries and Pituitary Adenomas Identified With Two-Dimensional Gel Electrophoresis and Mass Spectrometry.

ABSTRACT: Human prolactin (hPRL) plays multiple roles in growth, metabolism, development, reproduction, and immunoregulation, which is an important protein synthesized in a pituitary. Two-dimensional gel electrophoresis (2DE) is an effective method in identity of protein variants for in-depth insight into functions of that protein. 2DE, 2DE-based PRL-immunoblot, mass spectrometry, and bioinformatics were used to analyze hPRL variants in human normal (control; n = 8) pituitaries and in five subtypes of pituitary adenomas [NF^- (n = 3)-, FSH⁺ (n = 3)-, LH⁺ (n = 3)-, FSH⁺/LH⁺ (n = 3)-, and PRL⁺ (n = 3)-adenomas]. Six hPRL variants were identified with different isoelectric point (pI)-relati

SUBMITTER: Qian S 

PROVIDER: S-EPMC6121189 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature