Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense.

ABSTRACT: Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used. Recent studies have investigated suitable reference genes for some crustaceans under various conditions, but studies in Macrobrachium nipponense are currently lacking. In this study, we selected the following seven genes from among 35 commonly used housekeeping genes as candidate qPCR reference genes for temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S ribosomal RNA), EF-1α (elongation factor-1α), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (α-tubulin), β-act (β-actin),

SUBMITTER: Hu Y 

PROVIDER: S-EPMC6121487 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature