Understanding Ovarian Cancer: iTRAQ-Based Proteomics for Biomarker Discovery.

ABSTRACT: Despite many years of studies, ovarian cancer remains one of the top ten cancers worldwide. Its high mortality rate is mainly due to lack of sufficient diagnostic methods. For this reason, our research focused on the identification of blood markers whose appearance would precede the clinical manifestation of the disease. ITRAQ-tagging (isobaric Tags for Relative and Absolute Quantification) coupled with mass spectrometry technology was applied. Three groups of samples derived from patients with: ovarian cancer, benign ovarian tumor, and healthy controls, were examined. Mass spectrometry analysis allowed for highlighting the dysregulation of several proteins associated with ovarian cancer. Further validation of the obtained results indicated that five proteins (Serotransferrin, Amyloid A1,

SUBMITTER: Swiatly A 

PROVIDER: S-EPMC6121953 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature