Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing.

ABSTRACT:

Background

The World Health Organization recommends drug-susceptibility testing of Mycobacterium tuberculosis complex for all patients with tuberculosis to guide treatment decisions and improve outcomes. Whether DNA sequencing can be used to accurately predict profiles of susceptibility to first-line antituberculosis drugs has not been clear.

Methods

We obtained whole-genome sequences and associated phenotypes of resistance or susceptibility to the first-line antituberculosis drugs isoniazid, rifampin, ethambutol, and pyrazinamide for isolates from 16 countries across six continents. For each isolate, mutations associated with drug resistance and drug susceptibility were identified across nine genes, and individual phenotypes were predicted unless mutations of unknown associ

SUBMITTER: CRyPTIC Consortium and the 100,000 Genomes Project 

PROVIDER: S-EPMC6121966 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature