Linking Uncultivated Microbial Populations and Benthic Carbon Turnover by Using Quantitative Stable Isotope Probing.

ABSTRACT: Benthic environments harbor highly diverse and complex microbial communities that control carbon fluxes, but the role of specific uncultivated microbial groups in organic matter turnover is poorly understood. In this study, quantitative DNA stable isotope probing (DNA-qSIP) was used for the first time to link uncultivated populations of bacteria and archaea to carbon turnover in lacustrine surface sediments. After 1-week incubations in the dark with [¹³C]bicarbonate, DNA-qSIP showed that ammonia-oxidizing archaea (AOA) were the dominant active chemolithoautotrophs involved in the production of new organic matter. Natural ¹³C-labeled organic matter was then obtained by incubating sediments in the dark for 2.5 months with [¹³C]bicarbonate, followed by extract

SUBMITTER: Coskun OK 

PROVIDER: S-EPMC6122004 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature