Sanger and Next Generation Sequencing Approaches to Evaluate HIV-1 Virus in Blood Compartments.

ABSTRACT: The implementation of antiretroviral treatment combined with the monitoring of drug resistance mutations improves the quality of life of HIV-1 positive patients. The drug resistance mutation patterns and viral genotypes are currently analyzed by DNA sequencing of the virus in the plasma of patients. However, the virus compartmentalizes, and different T cell subsets may harbor distinct viral subsets. In this study, we compared the patterns of HIV distribution in cell-free (blood plasma) and cell-associated viruses (peripheral blood mononuclear cells, PBMCs) derived from ART-treated patients by using Sanger sequencing- and Next-Generation sequencing-based HIV assay. CD4⁺CD45RA^-RO⁺ memory T-cells were isolated from PBMCs using a BD FACSAria instrument. HIV pol (protease and

SUBMITTER: Arias A 

PROVIDER: S-EPMC6122037 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature