Genome alterations associated with improved transformation efficiency in Lactobacillus reuteri.

ABSTRACT:

Background

Lactic acid bacteria (LAB) are one of the microorganisms of choice for the development of protein delivery systems for therapeutic purposes. Although there are numerous tools to facilitate genome engineering of lactobacilli; transformation efficiency still limits the ability to engineer their genomes. While genetically manipulating Lactobacillus reuteri ATCC PTA 6475 (LR 6475), we noticed that after an initial transformation, several LR 6475 strains significantly improved their ability to take up plasmid DNA via electroporation. Our goal was to understand the molecular basis for how these strains acquired the ability to increase transformation efficiency.

Results

Strains generated after transformation of plasmids pJP067 and pJP042 increased their ability to transf

SUBMITTER: Ortiz-Velez L 

PROVIDER: S-EPMC6122466 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature