Linked read technology for assembling large complex and polyploid genomes.

ABSTRACT:

Background

Short read DNA sequencing technologies have revolutionized genome assembly by providing high accuracy and throughput data at low cost. But it remains challenging to assemble short read data, particularly for large, complex and polyploid genomes. The linked read strategy has the potential to enhance the value of short reads for genome assembly because all reads originating from a single long molecule of DNA share a common barcode. However, the majority of studies to date that have employed linked reads were focused on human haplotype phasing and genome assembly.

Results

Here we describe a de novo maize B73 genome assembly generated via linked read technology which contains ~ 172,000 scaffolds with an N50 of 89 kb that cover 50% of the genome. Based on comparisons t

SUBMITTER: Ott A 

PROVIDER: S-EPMC6122573 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature