Identification of novel reassortant mammalian orthoreoviruses from bats in Slovenia.

ABSTRACT:

Background

Recently, mammalian orthoreoviruses (MRVs) were detected for the first time in European bats, and the closely related strain SI-MRV01 was isolated from a child with severe diarrhoea in Slovenia. Genetically similar strains have also been reported from other mammals, which reveals their wide host distribution. The aim of this study was to retrospectively investigate the occurrence and genetic diversity of MRVs in bats in Slovenia, from samples obtained throughout the country in 2008 to 2010, and in 2012 and to investigate the occurrence of the novel SI-MRV01 MRV variant in Slovenian bats.

Results

The detection of MRVs in bat guano was based on broad-range RT-PCR and specific bat MRV real-time RT-PCR. Subsequently, MRV isolates were obtained from cell culture propag

SUBMITTER: Naglic T 

PROVIDER: S-EPMC6122641 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature