Magnetization of active inclusion bodies: comparison with centrifugation in repetitive biotransformations.

ABSTRACT:

Background

Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction cycle to another, as high centrifugation forces are needed in large volumes. The magnetization of inclusion bodies is a smart solution for large-scale applications, enabling an easier separation process using a magnetic field.

Results

Magnetically modified inclusion bodies of UDP-glucose pyrophosphorylase were recycled 50 times, in comparison, inclusion bodies of the same enzyme were inactivated during ten reaction cycles if they were recycled by centrifugation. In

SUBMITTER: Koszagova R 

PROVIDER: S-EPMC6122667 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature