Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase.

ABSTRACT:

Background

The recycling of agro-industrial wastes is at present limited by the availability of efficient and low-cost enzyme cocktails. The use of these materials as culture media to produce the enzymes can contribute to the profitability of the recycling process and to the circular economy. The aim of this work is the construction of a recombinant yeast strain efficient to grow in mixed whey (residue of cheese making) and beet molasses (residue of sugar manufacture) as culture medium, and to produce heterologous α-galactosidase, an enzyme with varied industrial applications and wide market.

Results

The gene MEL1, encoding the α-galactosidase of Saccharomyces cerevisiae, was integrated (four copies) in the LAC4 locus of the Kluyveromyces lactis industrial strain GG799. The

SUBMITTER: Alvarez-Cao ME 

PROVIDER: S-EPMC6122717 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature