Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue.

ABSTRACT:

Background

To study the expression pattern, localisation and potential clinical significance of aquaporin water channels (AQP) both in prostate cancer (PC) cell lines and in benign and malignant human prostate tissue.

Methods

The AQP transcript and protein expression of HPrEC, LNCaP, DU-145 and PC3 cell lines was investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence (IF) microscopy labelling. Immunohistochemistry (IHC) was performed to assess AQP protein expression in surgical specimens of benign prostatic hyperplasia as well as in PC. Tissue mRNA expression of AQPs was quantified by single-step reverse transcriptase quantitative polymerase chain reaction (qPCR). Relative gene expression was determined using the 40-ΔC_T

SUBMITTER: Brundl J 

PROVIDER: S-EPMC6122723 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature