Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay.

ABSTRACT:

Background

Muscovy duck parvovirus (MDPV) causes high mortality and morbidity in Muscovy ducks, with the pathogenesis of the virus still unknown in many respects. Specific MDPV detection is often rife with false positive results because of high identity at the genomic nucleotide level and antigenic similarity with goose parvovirus (GPV). The objective of this study was to develop a sensitive, highly specific, and repeatable TaqMan-based real-time PCR (qPCR) assay for facilitating the molecular detection of MDPV.

Results

The specific primers and probe were designed based on the conserved regions within MDPVs, but there was a variation in GPVs of the nonstructural (NS) genes after genetic comparison. After the optimization of qPCR conditions, the detection limit of this qPCR a

SUBMITTER: Wan C 

PROVIDER: S-EPMC6122767 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature