High fat diet disrupts endoplasmic reticulum calcium homeostasis in the rat liver.

ABSTRACT:

Background & aims

Disruption to endoplasmic reticulum (ER) calcium homeostasis has been implicated in obesity, however, the ability to longitudinally monitor ER calcium fluctuations has been challenging with prior methodologies. We recently described the development of a Gaussia luciferase (GLuc)-based reporter protein responsive to ER calcium depletion (GLuc-SERCaMP) and investigated the effect of a high fat diet on ER calcium homeostasis.

Methods

A GLuc-based reporter cell line was treated with palmitate, a free fatty acid. Rats intrahepatically injected with GLuc-SERCaMP reporter were fed a cafeteria diet or high fat diet. The liver and plasma were examined for established markers of steatosis and compared to plasma levels of SERCaMP activity.

Results

Palmitate ind

SUBMITTER: Wires ES 

PROVIDER: S-EPMC6122848 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature