Singlet oxygen modification abolishes voltage-dependent inactivation of the sea urchin spHCN channel.

ABSTRACT: Photochemically or metabolically generated singlet oxygen (¹O₂) reacts broadly with macromolecules in the cell. Because of its short lifetime and working distance, ¹O₂ holds potential as an effective and precise nanoscale tool for basic research and clinical practice. Here we investigate the modification of the spHCN channel that results from photochemically and chemically generated ¹O₂ The spHCN channel shows strong voltage-dependent inactivation in the absence of cAMP. In the presence of photosensitizers, short laser pulses transform the gating properties of spHCN by abolishing inactivation and increasing the macroscopic current amplitude. Alanine replacement of a histidine residue near the activation gate within the chann

SUBMITTER: Idikuda V 

PROVIDER: S-EPMC6122923 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature