<i>Drosophila</i> immune cells extravasate from vessels to wounds using Tre1 GPCR and Rho signaling.

ABSTRACT: Inflammation is pivotal to fight infection, clear debris, and orchestrate repair of injured tissues. Although Drosophila melanogaster have proven invaluable for studying extravascular recruitment of innate immune cells (hemocytes) to wounds, they have been somewhat neglected as viable models to investigate a key rate-limiting component of inflammation-that of immune cell extravasation across vessel walls-due to their open circulation. We have now identified a period during pupal development when wing hearts pulse hemolymph, including circulating hemocytes, through developing wing veins. Wounding near these vessels triggers local immune cell extravasation, enabling live imaging and correlative light-electron microscopy of these events in vivo. We show that RNAi knockdown of immune ce

SUBMITTER: Thuma L 

PROVIDER: S-EPMC6122984 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature