Shedding of CD16 disassembles the NK cell immune synapse and boosts serial engagement of target cells.

ABSTRACT: Natural Killer (NK) cells can engage multiple virally infected or tumor cells sequentially and deliver perforin for cytolytic killing of these targets. Using microscopy to visualize degranulation from individual NK cells, we found that repeated activation via the Fc receptor CD16 decreased the amount of perforin secreted. However, perforin secretion was restored upon subsequent activation via a different activating receptor, NKG2D. Repeated stimulation via NKG2D also decreased perforin secretion, but this was not rescued by stimulation via CD16. These different outcomes of sequential stimulation could be accounted for by shedding of CD16 being triggered by cellular activation. The use of pharmacological inhibitors and NK cells transfected to express a noncleavable form of CD16 revealed tha

SUBMITTER: Srpan K 

PROVIDER: S-EPMC6122987 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature