The nasal microbiome in asthma.

ABSTRACT: BACKGROUND:Nasal microbiota may influence asthma pathobiology. OBJECTIVE:We sought to characterize the nasal microbiome of subjects with exacerbated asthma, nonexacerbated asthma, and healthy controls to identify nasal microbiota associated with asthma activity. METHODS:We performed 16S ribosomal RNA sequencing on nasal swabs obtained from 72 primarily adult subjects with exacerbated asthma (n = 20), nonexacerbated asthma (n = 31), and healthy controls (n = 21). Analyses were performed using Quantitative Insights into Microbial (QIIME); linear discriminant analysis effect size (LEfSe); Phylogenetic Investigation of Communities by Reconstruction of Unobserved States; and Statistical Analysis of Metagenomic Profiles (PICRUSt); and Statistical Analysis of Metagenomic Profiles (STAMP). Species

SUBMITTER: Fazlollahi M 

PROVIDER: S-EPMC6123291 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature