The effects of monocytes on tumor cell extravasation in a 3D vascularized microfluidic model.

ABSTRACT: Metastasis is the leading cause of cancer-related deaths. Recent developments in cancer immunotherapy have shown exciting therapeutic promise for metastatic patients. While most therapies target T cells, other immune cells, such as monocytes, hold great promise for therapeutic intervention. In our study, we provide primary evidence of direct engagement between human monocytes and tumor cells in a 3D vascularized microfluidic model. We first characterize the novel application of our model to investigate and visualize at high resolution the evolution of monocytes as they migrate from the intravascular to the extravascular micro-environment. We also demonstrate their differentiation into macrophages in our all-human model. Our model replicates physiological differences between different monoc

SUBMITTER: Boussommier-Calleja A 

PROVIDER: S-EPMC6123301 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature