Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis.

ABSTRACT: A systematic interrogation of male germ cells is key to complete understanding of molecular mechanisms governing spermatogenesis and the development of new strategies for infertility therapies and male contraception. Here we develop an approach to purify all types of homogeneous spermatogenic cells by combining transgenic labeling and synchronization of the cycle of the seminiferous epithelium, and subsequent single-cell RNA-sequencing. We reveal extensive and previously uncharacterized dynamic processes and molecular signatures in gene expression, as well as specific patterns of alternative splicing, and novel regulators for specific stages of male germ cell development. Our transcriptomics analyses led us to discover discriminative markers for isolating round spermatids at specific stage

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6123400 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature