Single-neuronal cell culture and monitoring platform using a fully transparent microfluidic DEP device.

ABSTRACT: Dielectrophoresis using multi-electrode arrays allows a non-invasive interface with biological cells for long-term monitoring of electrophysiological parameters as well as a label-free and non-destructive technique for neuronal cell manipulation. However, experiments for neuronal cell manipulation utilizing dielectrophoresis have been constrained because dielectrophoresis devices generally function outside of the controlled environment (i.e. incubator) during the cell manipulation process, which is problematic because neurons are highly susceptible to the properties of the physiochemical environment. Furthermore, the conventional multi-electrode arrays designed to generate dielectrophoretic force are often fabricated with non-transparent materials that confound live-cell imaging. Here we p

SUBMITTER: Kim H 

PROVIDER: S-EPMC6123457 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature