Low genomic diversity of Legionella pneumophila within clinical specimens.

ABSTRACT: OBJECTIVES:Legionella pneumophila is the leading cause of Legionnaires' disease, a severe form of pneumonia acquired from environmental sources. Investigations of both sporadic cases and outbreaks rely mostly on analysis of a single to a few colony pick(s) isolated from each patient. However, because of the lack of data describing diversity within single patients, the optimal number of picks is unknown. Here, we investigated diversity within individual patients using sequence-based typing (SBT) and whole-genome sequencing (WGS). METHODS:Ten isolates of L. pneumophila were obtained from each of ten epidemiologically unrelated patients. SBT and WGS were undertaken, and single-nucleotide polymorphisms (SNPs) were identified between isolates from the same patient. RESULTS:The same sequence typ

SUBMITTER: David S 

PROVIDER: S-EPMC6123502 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature