High-resolution small RNA structures from exact nuclear Overhauser enhancement measurements without additional restraints.

ABSTRACT: RNA not only translates the genetic code into proteins, but also carries out important cellular functions. Understanding such functions requires knowledge of the structure and dynamics at atomic resolution. Almost half of the published RNA structures have been solved by nuclear magnetic resonance (NMR). However, as a result of severe resonance overlap and low proton density, high-resolution RNA structures are rarely obtained from nuclear Overhauser enhancement (NOE) data alone. Instead, additional semi-empirical restraints and labor-intensive techniques are required for structural averages, while there are only a few experimentally derived ensembles representing dynamics. Here we show that our exact NOE (eNOE) based structure determination protocol is able to define a 14-mer UUCG tetraloop

SUBMITTER: Nichols PJ 

PROVIDER: S-EPMC6123705 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature