Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes.

ABSTRACT: Generation of homogeneous populations of subtype-specific cardiomyocytes (CMs) derived from human induced pluripotent stem cells (iPSCs) and their comprehensive phenotyping is crucial for a better understanding of the subtype-related disease mechanisms and as tools for the development of chamber-specific drugs. The goals of this study were to apply a simple and efficient method for differentiation of iPSCs into defined functional CM subtypes in feeder-free conditions and to obtain a comprehensive understanding of the molecular, cell biological, and functional properties of atrial and ventricular iPSC-CMs on both the single-cell and engineered heart muscle (EHM) level. By a stage-specific activation of retinoic acid signaling in monolayer-based and well-defined culture, we showed that cardi

SUBMITTER: Cyganek L 

PROVIDER: S-EPMC6124434 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature