Layer-by-layer DNA films incorporating highly transfecting bioreducible poly(amido amine) and polyethylenimine for sequential gene delivery.

ABSTRACT:

Background

The layer-by-layer (LbL) assembly method offers a molecular level control of the amount and spatial distribution of bioactive molecules. However, successful clinical translation of LbL film technology will most certainly require a better understanding and control of not only the film assembly process, but also film disassembly kinetics in physiologic conditions.

Purpose

This work focuses on the understanding and control of degradation properties of LbL films for localized gene delivery.

Methods

Bioreducible poly(amido amine)s (PAAs) containing cystaminebisacrylamide (CBA), methylenebisacrylamide, and 5-amino-1-pentanol (APOL) were synthesized by Michael addition polymerization for the construction of bioreducible LbL films capable of sequential gene deliver

SUBMITTER: Xie L 

PROVIDER: S-EPMC6124456 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature