Concurrent cell type-specific isolation and profiling of mouse brains in inflammation and Alzheimer's disease.

ABSTRACT: Nonneuronal cell types in the CNS are increasingly implicated as critical players in brain health and disease. While gene expression profiling of bulk brain tissue is routinely used to examine alterations in the brain under various conditions, it does not capture changes that occur within single cell types or allow interrogation of crosstalk among cell types. To this end, we have developed a concurrent brain cell type acquisition (CoBrA) methodology, enabling the isolation and profiling of microglia, astrocytes, endothelia, and oligodendrocytes from a single adult mouse forebrain. By identifying and validating anti-ACSA-2 and anti-CD49a antibodies as cell surface markers for astrocytes and vascular endothelial cells, respectively, and using established antibodies to isolate microglia and o

SUBMITTER: Swartzlander DB 

PROVIDER: S-EPMC6124528 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature