Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney.

ABSTRACT: The mammalian kidney develops through reciprocal interactions between the ureteric bud and the metanephric mesenchyme to give rise to the entire collecting system and the nephrons. Most of our knowledge of the developmental regulators driving this process arises from the study of gene expression and functional genetics in mice and other animal models. In order to shed light on human kidney development, we have used single-cell transcriptomics to characterize gene expression in different cell populations, and to study individual cell dynamics and lineage trajectories during development. Single-cell transcriptome analyses of 6414 cells from five individual specimens identified 11 initial clusters of specific renal cell types as defined by their gene expression profile. Further subclustering

SUBMITTER: Menon R 

PROVIDER: S-EPMC6124540 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature