Neural differentiation, selection and transcriptomic profiling of human neuromesodermal progenitor-like cells <i>in vitro</i>.

ABSTRACT: Robust protocols for directed differentiation of human pluripotent cells are required to determine whether mechanisms operating in model organisms are relevant to our own development. Recent work in vertebrate embryos has identified neuromesodermal progenitors as a bipotent cell population that contributes to paraxial mesoderm and spinal cord. However, precise protocols for in vitro differentiation of human spinal cord progenitors are lacking. Informed by signalling in amniote embryos, we show here that transient dual-SMAD inhibition, together with retinoic acid (dSMADi-RA), provides rapid and reproducible induction of human spinal cord progenitors from neuromesodermal progenitor-like cells. Using CRISPR-Cas9 to engineer human embryonic stem cells with a GFP-reporter for neuromesode

SUBMITTER: Verrier L 

PROVIDER: S-EPMC6124542 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature