Three-dimensional induction of dorsal, intermediate and ventral spinal cord tissues from human pluripotent stem cells.

ABSTRACT: The spinal cord contains more than 20 distinct subclasses of neurons that form well-organized neural circuits capable of sensing the environment and generating motor behavior. Although recent studies have described the efficient in vitro generation of spinal motor neurons, the induction of the spinal cord as a whole tissue has not been achieved. In the present study, we demonstrate three-dimensional (3D) induction of dorsal spinal cord-like tissues from human pluripotent stem cells. Our 3D spinal cord induction (3-DiSC) condition recapitulates patterning of the developing dorsal spinal cord and enables the generation of four types of dorsal interneuron marker-positive cell populations. By activating Shh signaling, intermediate and ventral spinal cord-like tissues are successfully in

SUBMITTER: Ogura T 

PROVIDER: S-EPMC6124545 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature