A two-hybrid antibody micropattern assay reveals specific in cis interactions of MHC I heavy chains at the cell surface.

ABSTRACT: We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins that allow for location- and conformation-specific analysis by fluorescence microscopy. The assay is used to study the in cis interactions of MHC I proteins at the cell surface under controlled conditions and to define the involved protein conformations. Our results show that homotypic in cis interactions occur exclusively between MHC I free heavy chains, and we identify the dissociation of the light chain from the MHC I protein complex as a condition for MHC I in cis intera

SUBMITTER: Dirscherl C 

PROVIDER: S-EPMC6125123 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature