Heterotrimeric G-protein subunit Gα_i2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets.

ABSTRACT: Gα_i2 , a heterotrimeric G-protein subunit, regulates various cell functions including ion channel activity, cell differentiation, proliferation and apoptosis. Platelet-expressed Gα_i2 is decisive for the extent of tissue injury following ischemia/reperfusion. However, it is not known whether Gα_i2 plays a role in the regulation of platelet apoptosis, which is characterized by caspase activation, cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) translocation to the platelet surface. Stimulators of platelet apoptosis include thrombin and collagen-related peptide (CoRP), which are further known to enhance degranulation and activation of α_IIb β3-integrin and caspases. Using FACS analysis, we examined the impact of ago

SUBMITTER: Cao H 

PROVIDER: S-EPMC6125243 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature