Time course of liver mitochondrial function and intrinsic changes in oxidative phosphorylation in a rat model of sepsis.

ABSTRACT:

Background

Tissue ATP depletion and oxidative stress have been associated with the severe outcomes of septic shock. One of the compensatory mechanisms to alleviate the sepsis-induced mitochondrial dysfunction could be the increase in oxidative phosphorylation efficiency (ATP/O). We propose to study liver mitochondrial function and oxidative stress and the regulatory mechanism of mitochondrial oxidative phosphorylation efficiency in an animal model of sepsis.

Methods

We induced sepsis in rats by cecal ligation and perforation (CLP). Six, 24, or 36 h following CLP, we measured liver mitochondrial respiration, cytochrome c oxidase activity, and membrane permeability. We determine oxidative phosphorylation efficiency, by measuring ATP synthesis related to oxygen consumption at v

SUBMITTER: Eyenga P 

PROVIDER: S-EPMC6125261 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature