Histamine causes endothelial barrier disruption via Ca²⁺-mediated RhoA activation and tension at adherens junctions.

ABSTRACT: During inflammation, the disruption of the endothelial barrier leads to increased microvascular permeability. Whether tension along cell junctions contributes to histamine-induced endothelial barrier disruption remains unknown. Rapid Ca²⁺ influx induced by both histamine and thrombin was accompanied by endothelial barrier breakdown revealed as drop of transendothelial electric resistance in primary human microvascular endothelial cells. Interestingly, GLISA measurements revealed activation of RhoA but not inactivation of Rac1 at the time-point of barrier breakdown. FRET measurements showed activation of RhoA at intercellular junctions after both thrombin and histamine exposure. Breakdown coincided with increased stress fiber formation but not with translocation of vinculin, whic

SUBMITTER: Kugelmann D 

PROVIDER: S-EPMC6125323 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature