Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells.

ABSTRACT: The gravitational force has been constant throughout Earth's evolutionary history. Since the cell nucleus is subjected to permanent forces induced by Earth's gravity, we addressed the question, if gene expression homeostasis is constantly shaped by the gravitational force on Earth. We therefore investigated the transcriptome in force-free conditions of microgravity, determined the time frame of initial gravitational force-transduction to the transcriptome and assessed the role of cation channels. We combined a parabolic flight experiment campaign with a suborbital ballistic rocket experiment employing the human myelomonocytic cell line U937 and analyzed the whole gene transcription by microarray, using rigorous controls for exclusion of effects not related to gravitational force and cross-

SUBMITTER: Thiel CS 

PROVIDER: S-EPMC6125427 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature