Gata4-Dependent Differentiation of c-Kit⁺-Derived Endothelial Cells Underlies Artefactual Cardiomyocyte Regeneration in the Heart.

ABSTRACT:

Background

Although c-Kit⁺ adult progenitor cells were initially reported to produce new cardiomyocytes in the heart, recent genetic evidence suggests that such events are exceedingly rare. However, to determine if these rare events represent true de novo cardiomyocyte formation, we deleted the necessary cardiogenic transcription factors Gata4 and Gata6 from c-Kit-expressing cardiac progenitor cells.

Methods

Kit allele-dependent lineage tracing and fusion analysis were performed in mice following simultaneous Gata4 and Gata6 cell type-specific deletion to examine rates of putative de novo cardiomyocyte formation from c-Kit⁺ cells. Bone marrow transplantation experiments were used to define the contribution of Kit allele-derived hematopoietic cells versu

SUBMITTER: Maliken BD 

PROVIDER: S-EPMC6125755 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature