SOX2OT knockdown derived changes in mitotic regulatory gene network of cancer cells.

ABSTRACT:

Background

SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA, over-expressed in human tumor tissues and embryonic cells. Evidences support its function in the cell cycle; however there is no clear mechanism explaining its function in cell proliferation regulation. Here we investigated cancer cell response to SOX2OT knockdown by RNA sequencing.

Methods

SOX2OT expression was inhibited by siRNA in two cancer cell lines (A549, U-87 MG), then the RNA of treated cells were used for the cDNA library synthesis and RNA sequencing. The differentially expressed genes were used for functional enrichment and the gene expression network was analyzed to find the most relevant biological process with SOX2OT function. Furthermore, the expression change of candidate genes was meas

SUBMITTER: Saghaeian Jazi M 

PROVIDER: S-EPMC6126007 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature