High-resolution ISR amplicon sequencing reveals personalized oral microbiome.

ABSTRACT:

Background

Sequencing of the 16S rRNA gene has been the standard for studying the composition of microbial communities. While it allows identification of bacteria at the level of species, this method does not usually provide sufficient information to resolve communities at the sub-species level. Species-level resolution is not adequate for studies of transmission or stability or for exploring subspecies variation in disease association. Strain level analysis using whole metagenome shotgun sequencing has significant limitations that can make it unsuitable for large-scale studies. Achieving sufficient depth of sequencing can be cost-prohibitive, and even with adequate coverage, deconvoluting complex communities such as the oral microbiota is computationally very challenging. Thus, th

SUBMITTER: Mukherjee C 

PROVIDER: S-EPMC6126016 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature