The CO₂-dependence of Brucella ovis and Brucella abortus biovars is caused by defective carbonic anhydrases.

ABSTRACT: Brucella bacteria cause brucellosis, a major zoonosis whose control requires efficient diagnosis and vaccines. Identification of classical Brucella spp. has traditionally relied on phenotypic characterization, including surface antigens and 5-10% CO₂ necessity for growth (CO₂-dependence), a trait of Brucella ovis and most Brucella abortus biovars 1-4 strains. Although molecular tests are replacing phenotypic methods, CO₂-dependence remains of interest as it conditions isolation and propagation and reflects Brucella metabolism, an area of active research. Here, we investigated the connection of CO₂-dependence and carbonic anhydrases (CA), the enzymes catalyzing the hydration of CO₂ to the bicarbonate used by anaplerotic and biosynthetic

SUBMITTER: Perez-Etayo L 

PROVIDER: S-EPMC6126018 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature