Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells.

ABSTRACT:

Background

Adipose-tissue stem cells (ASCs) are subject of intensive research since their successful use in regenerative therapy. The drawback of ASCs is that they may serve as stroma for cancer cells and assist tumor progression. It is disquieting that ASCs frequently undergo genetic and epigenetic changes during their in vitro propagation. In this study, we describe the polyploidization of murine ASCs and the accompanying phenotypical, gene expressional and functional changes under long term culturing.

Methods

ASCs were isolated from visceral fat of C57BL/6 J mice, and cultured in vitro for prolonged time. The phenotypical changes were followed by microscopy and flow cytometry. Gene expressional changes were determined by differential transcriptome analysis and changes in

SUBMITTER: Fajka-Boja R 

PROVIDER: S-EPMC6126028 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature